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bioRxiv · 10.1101/837682

microRNA seedless sites attenuate strong-seed-site-mediated target repression

Wang, X.·Tian, J.·Cui, P.·Mastriano, S.·Zhang, D.·Zhao, H.·Lu, H.·Ding, Y.·Lu, J.
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Abstract

MicroRNAs (miRNAs) regulate protein-coding gene expression primarily through cognitive binding sites in the 3 untranslated regions (3' UTRs). Seed sites are sequences in messenger RNAs (mRNAs) that form perfect Watson-Crick base-paring with a miRNAs seed region, which can effectively reduce mRNA abundance and/or repress protein translation. Some seedless sites, which do no form perfect seed-pairing with a miRNA, can also lead to target repression, often with lower efficacy. Here we report the surprising finding that when seedless sites and seed sites are co-present in the same 3UTR, seedless sites attenuate strong-seed-site-mediated target suppression, independent of 3' UTR length. This attenuation effect is detectable in >70% of transcriptomic datasets examined, in which specific miRNAs are experimentally increased or decreased. The attenuation effect is confirmed by 3UTR reporter assays and mediated through base-pairing between miRNA and seedless sites. Furthermore, this seedless-site-based attenuation effect could affect seed sites of the same miRNA or another miRNA, thus partially explaining the variability in target suppression and miRNA-mediated gene upregulation. Our findings reveal an unexpected principle of miRNA-mediated gene regulation, and could impact the understanding of many miRNA-regulated biological processes.

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Wang, X., Tian, J., Cui, P., Mastriano, S., Zhang, D., Zhao, H., Lu, H., Ding, Y., Lu, J.. 2019-11-11. microRNA seedless sites attenuate strong-seed-site-mediated target repression. https://doi.org/10.1101/837682

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