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bioRxiv · 10.1101/836023

The histone modification reader ZCWPW1 links histone methylation to repair of PRDM9-induced meiotic double stand breaks

Huang, T.·Yuan, S.·Li, M.·Yu, X.·Zhang, J.·Yin, Y.·Liu, C.·Gao, L.·Li, W.·Liu, J.·Chen, Z.-J.·Liu, H.
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Abstract

The histone modification writer PRDM9 has been shown to deposit H3K4me3 and H3K36me3 at future double-strand break (DSB) sites during the very early stages of meiosis, but the reader of these marks remains unclear. Here, we demonstrate that ZCWPW1 is an H3K4me3 reader that is required for DSB repair and synapsis in mouse testes. We generated H3K4me3 reader-dead ZCWPW1 mutant mice and found that their spermatocytes were arrested at the pachytene-like stage, which phenocopies the Zcwpw1 knock-out mice. Based on various ChIP-seq and immunofluorescence analyses using several mutants, we found that ZCWPW1s occupancy on chromatin is strongly promoted by the histone-modification activity of PRDM9. ZCWPW1 localizes to DMC1-labelled hotspots in a largely PRDM9-dependent manner, where it facilitates completion of synapsis by mediating the DSB repair process. In sum, our study demonstrates the function of ZCWPW1 that acts as part of the selection system for epigenetics-based recombination hotspots in mammals.

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Huang, T., Yuan, S., Li, M., Yu, X., Zhang, J., Yin, Y., Liu, C., Gao, L., Li, W., Liu, J., Chen, Z.-J., Liu, H.. 2019-11-08. The histone modification reader ZCWPW1 links histone methylation to repair of PRDM9-induced meiotic double stand breaks. https://doi.org/10.1101/836023

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