Dynamic Palmitoylation Events Following T-Cell Receptor Signaling

Morrison, E.; Wegner, T.; Zucchetti, A. E.; Alvaro-Benito, M.; Zheng, A.; Kliche, S.; Krause, E.; Bruegger, B.; Hivroz, C.; Freund, C.

doi:10.1101/831388

bioRxiv · 10.1101/831388

Dynamic Palmitoylation Events Following T-Cell Receptor Signaling

Morrison, E.·Wegner, T.·Zucchetti, A. E.·Alvaro-Benito, M.·Zheng, A.·Kliche, S.·Krause, E.·Bruegger, B.·Hivroz, C.·Freund, C.

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Abstract

Palmitoylation is the reversible addition of palmitate to cysteine via a thioester linkage. Following stimulation of the T-cell receptor we find a number of proteins are newly palmitoylated, including those involved in vesicle-mediated transport and Ras signal transduction. Among these stimulation-dependent palmitoylation targets are the v-SNARE VAMP7, important for docking of vesicular LAT during TCR signaling, and the largely undescribed palmitoyl acyltransferase DHHC18 that is expressed in two isoforms in T cells. Using our newly developed On-Plate Palmitoylation Assay (OPPA), we show DHHC18 is capable of palmitoylating VAMP7 at Cys183. Cellular imaging shows that the palmitoylation-deficient protein fails to be retained at the Golgi.

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Morrison, E., Wegner, T., Zucchetti, A. E., Alvaro-Benito, M., Zheng, A., Kliche, S., Krause, E., Bruegger, B., Hivroz, C., Freund, C.. 2019-11-05. Dynamic Palmitoylation Events Following T-Cell Receptor Signaling. https://doi.org/10.1101/831388

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