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bioRxiv · 10.1101/830323

Mitochondrial clearance of Ca2+ controls insulin secretion

Abstract

Transport of Ca2+ from the cytosol to the mitochondrial matrix of insulin-secreting pancreatic {beta}-cells facilitates nutrient-mediated insulin secretion. However, the underlying mechanism is unclear. The establishment of the molecular identity of the mitochondrial Ca2+ uniporter (MCU) and associated proteins has allowed mitochondrial Ca2+ transport to be modified in intact cells. We examined the consequences of deficiency of the accessory protein, MICU2, in rat and human insulin-secreting cell lines as well as in mouse islets. Glucose-induced mitochondrial Ca2+ elevation and inner membrane hyperpolarization were reduced, together with cytosolic ATP/ADP-ratios and insulin secretion. Insulin secretion in Micu2 knock out mice was attenuated in vitro as well as in vivo. While KCl-evoked sub-plasmalemmal Ca2+ increases were more pronounced, the global cytosolic Ca2+ response was, surprisingly, diminished in MICU2-deficient cells. These findings were supported by selective inhibition of mitochondrial Ca2+ uptake by mitochondrial depolarization. It is concluded that mitochondrial Ca2+ transport plays an additional and hitherto unrecognized role in stimulated {beta}-cells by regulating net Ca2+ entry across the plasma membrane. This is likely accounted for by clearing of sub-plasmalemmal Ca2+ levels by mitochondria located near the plasma membrane.

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BibTeXRIS

Mulder, H., Vishnu, N., Hamilton, A., Bagge, A., Wernersson, A., Cowan, E., Barnard, H., Sancak, Y., Kamer, K. J., Spegel, P., Fex, M., Tengholm, A., Mootha, V., Nicholls, D. G.. 2019-11-05. Mitochondrial clearance of Ca2+ controls insulin secretion. https://doi.org/10.1101/830323

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