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bioRxiv · 10.1101/826230

TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology

Millacura, F. A.·Li, M.·Valenzuela-Ortega, M. A.·French, C.
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Abstract

While synthetic biology represents a promising approach to solve real-world problems, the use of genetically modified organisms is a cause of legal and environmental concerns. Cell-free systems have emerged as a possible solution but much work is needed to optimize their functionality and simplify their usage for Synthetic Biology. Here we present TXO, transcription-only genetic circuits, independent of translation or post-translation maturation. RNA aptamers are used as reaction output allowing the generation of fast, reliable and simple-to-design transcriptional units. TXO cell-free reactions and their possible applications are a promising new tool for fast and simple bench-to-market genetic circuit and biosensor applications.

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Millacura, F. A., Li, M., Valenzuela-Ortega, M. A., French, C.. 2019-10-31. TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology. https://doi.org/10.1101/826230

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