ROS production by localized SCHENGEN receptor module drives lignification at subcellular precision

Fujita, S.; Bellis, D. D.; Edel, K. H.; Köster, P.; Andersen, T. G.; Schmid-Siegert, E.; Tendon, V. D.; Pfister, A.; Marhavy, P.; Ursache, R.; Doblas, V. G.; Daraspe, J.; Creff, A.; Ingram, G.; Kudla, J.; Geldner, N.

doi:10.1101/818997

bioRxiv · 10.1101/818997

ROS production by localized SCHENGEN receptor module drives lignification at subcellular precision

Fujita, S.·Bellis, D. D.·Edel, K. H.·Köster, P.·Andersen, T. G.·Schmid-Siegert, E.·Tendon, V. D.·Pfister, A.·Marhavy, P.·Ursache, R.·Doblas, V. G.·Daraspe, J.·Creff, A.·Ingram, G.·Kudla, J.·Geldner, N.

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Abstract

Production of reactive-oxygen species (ROS) by NADPH oxidases (NOXs) impacts many processes in animals and plants and many plant receptor pathways involve rapid, NOX-dependent increases of ROS. Yet, their general reactivity has made it challenging to pinpoint the precise role and direct cellular targets of ROS. A well-understood ROS target in plants are lignin peroxidases in the cell wall. Lignin can be deposited with exquisite spatial control, but the underlying mechanisms have remained elusive. Here we establish a full kinase signaling relay that exerts direct, spatial control over ROS production and lignification within the cell wall. We show that polar localization of a single kinase component is crucial for pathway function. Our data indicates that an intersection of more broadly localized components allows for micrometer-scale precision of lignification and that this system is triggered through initiation of ROS production as a critical peroxidase co-substrate.

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Fujita, S., Bellis, D. D., Edel, K. H., Köster, P., Andersen, T. G., Schmid-Siegert, E., Tendon, V. D., Pfister, A., Marhavy, P., Ursache, R., Doblas, V. G., Daraspe, J., Creff, A., Ingram, G., Kudla, J., Geldner, N.. 2019-10-25. ROS production by localized SCHENGEN receptor module drives lignification at subcellular precision. https://doi.org/10.1101/818997

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