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bioRxiv · 10.1101/816660

jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

Mohr, M. A.·Bushey, D.·Aggarwal, A.·Marvin, J.·Jimenez Marquez, E.·Liang, Y.·Patel, R.·Macklin, J.·Lee, C.-Y.·Kim, D.·Wong, A. M.·Looger, L. L.·Schreiter, E. R.·Podgorski, K.
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Abstract

State-of-the-art GFP-based calcium indicators do not undergo efficient two-photon excitation at wavelengths above 1000 nm, for which inexpensive and powerful industrial femtosecond lasers are available. Here we report jYCaMP1, a yellow variant of jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators.

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Mohr, M. A., Bushey, D., Aggarwal, A., Marvin, J., Jimenez Marquez, E., Liang, Y., Patel, R., Macklin, J., Lee, C.-Y., Kim, D., Wong, A. M., Looger, L. L., Schreiter, E. R., Podgorski, K.. 2019-10-25. jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths. https://doi.org/10.1101/816660

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