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bioRxiv · 10.1101/803205

Accurate cell tracking and lineage construction in live-cell imaging experiments with deep learning

Abstract

While live-cell imaging is a powerful approach to studying the dynamics of cellular systems, converting these imaging data into quantitative, single-cell records of cellular behavior has been a longstanding challenge. Deep learning methods have proven capable of performing cell segmentation--a critical task for analyzing live-cell imaging data--but their performance in cell tracking has been limited by a lack of dynamic datasets with temporally consistent single-cell labels. We bridge this gap through the integrated development of labeling and deep learning methodology. We present a new framework for scalable, human-in-the-loop labeling of live-cell imaging movies, which we use to label a large collection of movies of fluorescently labeled cell nuclei. We use these data to create a new deep-learning-based cell-tracking method that achieves state-of-the-art performance in cell tracking. We have made all of the data, code, and software publicly available with permissive open-source licensing through the DeepCell projects web portal https://deepcell.org.

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BibTeXRIS

Moen, E., Borba, E., Miller, G., Schwartz, M., Bannon, D., Koe, N., Camplisson, I., Kyme, D., Pavelchek, C., Price, T., Kudo, T., Pao, E., Graf, W., Van Valen, D. A.. 2019-10-13. Accurate cell tracking and lineage construction in live-cell imaging experiments with deep learning. https://doi.org/10.1101/803205

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