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bioRxiv · 10.1101/802728

Critical role for Slam/SAP signaling in the thymic developmental programming of IL-17- and IFN-γ-producing γδ T cells

Abstract

During thymic development, {gamma}{delta} T cells commit to either an IFN-{gamma}- or an IL-17-producing phenotype through mechanisms that remain unclear. Here, we investigated whether the SLAM/SAP signaling pathway played a role in the functional programming of thymic {gamma}{delta} T cells. Characterization of SLAM family receptor expression revealed that thymic {gamma}{delta} T cell subsets were each marked by distinct co-expression profiles of SLAMF1, SLAMF4, and SLAMF6. In the thymus, immature CD24hi V{gamma}1 and V{gamma}4 {gamma}{delta} T cells were largely contained within a SLAMF1+SLAMF6+ double positive (DP) population, while mature CD24low subsets were either SLAMF1+ or SLAMF6+ single positive (SP) cells. In the periphery, SLAMF1 and SLAMF6 expression on V{gamma}1, V{gamma}4, and V{gamma}6 T cells distinguished IL-17- and IFN-{gamma}-producing subsets, respectively. Disruption of SLAM family receptor signaling through deletion of SAP resulted in impaired thymic {gamma}{delta} T cell maturation at the CD24hiSLAMF1+SLAMF6+ DP stage that was associated with a decreased frequency of CD44+ROR{gamma}t+ {gamma}{delta} T cells. These defects were in turn associated with impaired {gamma}{delta} T cell IL-17 and IFN-{gamma} production in both the thymus as well as in peripheral tissues. The role for SAP was subset-specific, as V{gamma}1, V{gamma}4, V{gamma}5, but not V{gamma}6 subsets were SAP-dependent. Together, these data suggest that the SLAM/SAP signaling pathway regulates a critical checkpoint in the functional programming of IL-17 and IFN-{gamma}-producing {gamma}{delta} T cell subsets during thymic development.

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BibTeXRIS

Dienz, O., DeVault, V. L., Musial, S. C., Mistri, S. K., Mei, L., Baraev, A., Dragon, J. A., Krementsov, D., Veillette, A., Boyson, J. E.. 2019-10-11. Critical role for Slam/SAP signaling in the thymic developmental programming of IL-17- and IFN-γ-producing γδ T cells. https://doi.org/10.1101/802728

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