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bioRxiv · 10.1101/795583

Calcium Imaging Reveals Host-Graft Synaptic Network Formation in Spinal Cord Injury

Ceto, S.·Sekiguchi, K. J.·Takashima, Y.·Nimmerjahn, A.·Tuszynski, M. H.
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Abstract

Neural stem/progenitor cell grafts integrate into sites of spinal cord injury (SCI) and form anatomical and electrophysiological neuronal relays across lesions. To determine how grafts become synaptically organized and connect with host systems, we performed calcium imaging of neural progenitor cell grafts within sites of SCI, using both in vivo imaging and spinal cord slices. Stem cell grafts organize into localized synaptic networks that are spontaneously active. Following optogenetic stimulation of host corticospinal tract axons regenerating into grafts, distinct and segregated neuronal networks respond throughout the graft. Moreover, optogenetic stimulation of graft axons extending out from the lesion into the denervated spinal cord also trigger responses in local host neuronal networks. In vivo imaging reveals that behavioral stimulation of host elicits focal synaptic responses within grafts. Thus, remarkably, neural progenitor cell grafts form functional synaptic subnetworks in patterns paralleling the normal spinal cord.

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Ceto, S., Sekiguchi, K. J., Takashima, Y., Nimmerjahn, A., Tuszynski, M. H.. 2019-10-07. Calcium Imaging Reveals Host-Graft Synaptic Network Formation in Spinal Cord Injury. https://doi.org/10.1101/795583

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