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bioRxiv · 10.1101/783837

Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity and functioning

He, L.·Koostra, R.·Das, R.·Oudejans, E.·van Leen, E. V.·Ziegler, J.·Portegies, S.·de Haan, B.·van Regteren Altena, A. S.·Stucchi, R.·Altelaar, A. F. M.·Wieser, S.·Krieg, M.·Hoogenraad, C. C.·Harterink, M.
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Abstract

Neurons are among the most highly polarized cell types. They possess structurally and functionally different processes, axon and dendrites, to mediate information flow through the nervous system. Although it is well known that the microtubule cytoskeleton has a central role in establishing neuronal polarity, how its specific organization is established and maintained is little understood.\n\nUsing the in vivo model system Caenorhabditis elegans, we found that the highly conserved UNC-119 protein provides a link between the membrane-associated Ankyrin (UNC-44) and the microtubule-associated CRMP (UNC-33). Together they form a periodic membrane-associated complex that anchors axonal and dendritic microtubule bundles to the cell cortex. This anchoring is critical to maintain microtubule organization by opposing kinesin-1 powered microtubule sliding. Disturbing this molecular complex alters neuronal polarity and causes strong developmental defects of the nervous system leading to severely paralyzed animals.

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He, L., Koostra, R., Das, R., Oudejans, E., van Leen, E. V., Ziegler, J., Portegies, S., de Haan, B., van Regteren Altena, A. S., Stucchi, R., Altelaar, A. F. M., Wieser, S., Krieg, M., Hoogenraad, C. C., Harterink, M.. 2019-09-26. Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity and functioning. https://doi.org/10.1101/783837

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