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bioRxiv · 10.1101/777664

Endosome-Mediated Epithelial Remodeling Downstream of Hedgehog/Gli Is Required for Tracheoesophageal Separation

Abstract

The trachea and esophagus arise from the separation of a common foregut tube during early fetal development. Mutations in key signaling pathways such as Hedgehog (HH)/Gli can disrupt tracheoesophageal (TE) morphogenesis and cause life-threatening birth defects (TEDs), however the underlying cellular mechanisms are unknown. Here we use mouse and Xenopus to define the HH/Gli-dependent processes orchestrating TE morphogenesis. We show that downstream of Gli the Foxf1+ splanchnic mesenchyme promotes medial constriction of the foregut at the boundary between the presumptive Sox2+ esophageal and Nkx2-1+ tracheal epithelium. We identify a unique boundary epithelium co-expressing Sox2 and Nkx2-1 that fuses to form a transient septum. Septum formation and resolution into distinct trachea and esophagus requires endosome-mediated epithelial remodeling involving the small GTPase Rab11, and localized extracellular matrix degradation. These are disrupted in Gli-deficient embryos. This work provides a new mechanistic framework for TE morphogenesis and informs the cellular basis of human TEDs.\n\nHighlight bullet pointsO_LIThe Sox2+ esophagus and Nkx2-1+ trachea arise from the separation of a single foregut tube through a series of cellular events conserved in mouse and Xenopus\nC_LIO_LITracheoesophageal morphogenesis initiates with HH/Gli-dependent medial constriction of the gut tube mesenchyme at the Sox2-Nkx2-1 border\nC_LIO_LIThe foregut epithelial walls fuse forming a transient septum co-expressing Sox2 and Nkx2-1\nC_LIO_LIDownstream of HH/Gli Rab11-dependent endosome-mediated epithelial remodeling and localized extracellular matrix degradation separate the esophagus and trachea\nC_LIO_LIHH/Gli mutations reveal the cellular basis of tracheoesophageal birth defects\nC_LI

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Nasr, T., Mancini, P., Rankin, S. A., Edwards, N. A., Agricola, Z. N., Kenny, A. P., Kinney, J. L., Daniels, K., Vardanyan, J., Han, L., Trisno, S. L., Wells, J. M., Cha, S.-W., Kofron, M., Zorn, A.. 2019-09-21. Endosome-Mediated Epithelial Remodeling Downstream of Hedgehog/Gli Is Required for Tracheoesophageal Separation. https://doi.org/10.1101/777664

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