Prospective evaluation of beta-lactamase detection in penicillin susceptible Staphylococcus aureus by interpretation of the penicillin disc edge

Henderson, A.; Whiley, D.; Francis, F.; Engler, C.; Glover, M.; Douglas, J.; Gore, L.; Harris, P. N. A.; Paterson, D.; Nimmo, G. R.; Norton, R.

doi:10.1101/776880

bioRxiv · 10.1101/776880

Prospective evaluation of beta-lactamase detection in penicillin susceptible Staphylococcus aureus by interpretation of the penicillin disc edge

Henderson, A.·Whiley, D.·Francis, F.·Engler, C.·Glover, M.·Douglas, J.·Gore, L.·Harris, P. N. A.·Paterson, D.·Nimmo, G. R.·Norton, R.

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Abstract

Penicillin susceptible Staphylococcus aureus (PSSA) may occasionally be encountered as a cause of complicated S. aureus infection, such as endocarditis or bloodstream infections. Clinicians may choose to treat these patients with penicillin over a semi-synthetic penicillin derivative, such as flucloxacillin or oxacillin, due to a favourable Pk/Pd profile. In this study, we prospectively evaluated the penicillin disc (1-IU) method for detection of blaZ, with interpretation of the penicillin edge according to EUCAST recommendations. 472 PSSA isolates were collected between September 2014 to December 2015 from three clinical microbiology laboratories in Queensland, Australia. Initial antimicrobial susceptibility testing was performed by the Vitek 2 system. Real-time PCR for blaZ was performed following phenotypic testing with the 1-IU penicillin disc and the PCR used as the gold standard for detection of penicillinase. The prevalence of blaZ amongst the isolates was 7%. The sensitivity, specificity, positive predictive value and negative predictive value of the penicillin disc method was 97%, 95%, 61% and 100% when compared to blaZ PCR. In summary, the penicillin disc zone size and edge interpretation is a reliable method for detection of blaZ in S. aureus isolates that otherwise test susceptible to penicillin by Vitek 2 AST.

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Henderson, A., Whiley, D., Francis, F., Engler, C., Glover, M., Douglas, J., Gore, L., Harris, P. N. A., Paterson, D., Nimmo, G. R., Norton, R.. 2019-09-26. Prospective evaluation of beta-lactamase detection in penicillin susceptible Staphylococcus aureus by interpretation of the penicillin disc edge. https://doi.org/10.1101/776880

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