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bioRxiv · 10.1101/768986

LncRNA MIR4435-2HG mediates cisplatin resistance in HCT116 cells by regulating Nrf2 and HO-1

Zhong, X.·Luo, P.·Wu, S.·Zhou, C.·Yuan, X.·Li, H.·Chen, J.·Tian, Y.·Qiu, Y.·Ping Luo,ShuGui Wu,ChaoMing Zhou,Xia Yuan,HongMi Li,JinPing Chen,YunFei Tian,Yang Qiu,XiaoMing Zhong,
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Abstract

PURPOSECisplatin resistance is still a serious problem in clinic. However, the underlying mechanism remains unclear. In this study investigated the drug resistance of cisplatin by the cisplatin resistance cell line HCT116R.\n\nRESULTSIn this study, we found that LncRNA MIR4435-2HG level dramatically increased in the cisplatin resistance cell line HCT116R. Knockdown of MIR4435-2HG in HCR116R significantly restored the sensitivity to cisplatin, inhibited cell proliferation and promoted cell apoptosis. Furthermore, Nrf2 and HO-1 mRNA level, as the critical molecular of the oxidative stress pathway, was inhibited by the siRNA targeting to MIR4435-2HG, displaying MIR4435-2HG-mediated cisplatin resistance through the Nrf2/HO-1 pathway.\n\nCONCLUSIONOur findings demonstrated that LncRNA MIR4435-2HG as a main factor could drive the cisplatin resistance of HCT116.

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Zhong, X., Luo, P., Wu, S., Zhou, C., Yuan, X., Li, H., Chen, J., Tian, Y., Qiu, Y., Ping Luo,ShuGui Wu,ChaoMing Zhou,Xia Yuan,HongMi Li,JinPing Chen,YunFei Tian,Yang Qiu,XiaoMing Zhong,. 2019-09-13. LncRNA MIR4435-2HG mediates cisplatin resistance in HCT116 cells by regulating Nrf2 and HO-1. https://doi.org/10.1101/768986

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