ECM-body: A cell-free 3D biomimetic scaffold derived from intact planarian body

Sonpho, E.; Wootthichairangsan, C.; Ishida, M.; Inoue, T.; Agata, K.; Maleehuan, A.; Charngkaew, K.; Chomanee, N.; Moonsom, S.; Wongtrakoongate, P.; Chairoungdua, A.; Ounjai, P.

doi:10.1101/763714

bioRxiv · 10.1101/763714

ECM-body: A cell-free 3D biomimetic scaffold derived from intact planarian body

Sonpho, E.·Wootthichairangsan, C.·Ishida, M.·Inoue, T.·Agata, K.·Maleehuan, A.·Charngkaew, K.·Chomanee, N.·Moonsom, S.·Wongtrakoongate, P.·Chairoungdua, A.·Ounjai, P.

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Abstract

Extracellular matrix (ECM) plays key roles in shaping fates of stem cells, not only by providing suitable niche but also by mediating physical and biochemical cues. Despite intensive investigations on regeneration, the roles of ECM on fate determination of stem cells in animal with great regenerative potency, such as planarian, remained unclear. Here, we developed a method to decellularizing and isolating extracellular matrix from planarians. Although the isolated scaffold appears translucent, it contains all the internal features, resembling the structure of intact planarian, and which we thus called \"ECM-body\". Nuclear staining demonstrated that ECM-body contains very little or no cell remained. Histological sections displayed a well-preserved morphological integrity of the specimen. Scanning electron microscope showed porous surface on ECM-body, potentially suitable for housing cells. Furthermore, our preliminary experiment suggested that ECM-body can be utilized as biomimetic scaffold for cell culture as it may support survival of injected neoblasts.

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Sonpho, E., Wootthichairangsan, C., Ishida, M., Inoue, T., Agata, K., Maleehuan, A., Charngkaew, K., Chomanee, N., Moonsom, S., Wongtrakoongate, P., Chairoungdua, A., Ounjai, P.. 2019-09-11. ECM-body: A cell-free 3D biomimetic scaffold derived from intact planarian body. https://doi.org/10.1101/763714

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