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bioRxiv · 10.1101/744144

Failure to clear developmental apoptosis contributes to the pathology of RNASET2-deficient leukoencephalopathy.

Hamilton, N.·Rutherford, H. A.·Isles, H. M.·Petts, J. J.·Henneke, M.·Gaertner, J.·Dunning, M.·Renshaw, S. A.
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Abstract

The contribution of microglia in neurological disorders is emerging as a leading driver rather than a consequence of pathology. RNAseT2-deficient leukoencephalopathy is a severe childhood white matter disorder affecting patients in their first year of life and mimics a cytomegalovirus brain infection. The early onset and resemblance of the symptoms to an immune response suggest an inflammatory and embryonic origin of the pathology. In this study, we identify deficient microglia as an early marker of pathology. Using the ex utero development and the optical transparency of an rnaset2-deficient zebrafish model, we found that dysfunctional microglia fail to clear apoptotic neurons during brain development. This was associated with increased number of apoptotic cells and behavioural defects lasting into adulthood. This zebrafish model recapitulates all aspect of the human disease to be used as a robust preclinical model. Using microglia-specific depletion and rescue experiments, we identified microglia as potential drivers of the pathology and highlight tissue-specific approaches as future therapeutic avenues.

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Hamilton, N., Rutherford, H. A., Isles, H. M., Petts, J. J., Henneke, M., Gaertner, J., Dunning, M., Renshaw, S. A.. 2019-08-22. Failure to clear developmental apoptosis contributes to the pathology of RNASET2-deficient leukoencephalopathy.. https://doi.org/10.1101/744144

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