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bioRxiv · 10.1101/743401

Distinct neuronal subtypes of the lateral habenula differentially target ventral tegmental area dopamine neurons

Wallace, M. L.·Huang, K. W.·Hochbaum, D.·Hyun, M.·Radeljic, G.·Sabatini, B. L.
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Abstract

The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of the habenula remain unknown. Here we use high-throughput single-cell transcriptional profiling, monosynaptic retrograde tracing, and multiplexed FISH to characterize the cells of the mouse habenula. We find 5 subtypes of neurons in the medial habenula (MHb) that are organized into anatomical subregions. In the LHb we describe 4 neuronal subtypes and show that they differentially target dopaminergic and GABAergic cells in the ventral tegmental area (VTA). These data provide a valuable resource for future study of habenular function and dysfunction and demonstrate neuronal subtype specificity in the LHb-VTA circuit.

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Wallace, M. L., Huang, K. W., Hochbaum, D., Hyun, M., Radeljic, G., Sabatini, B. L.. 2019-08-21. Distinct neuronal subtypes of the lateral habenula differentially target ventral tegmental area dopamine neurons. https://doi.org/10.1101/743401

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