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bioRxiv · 10.1101/737254

Revaluation of magnetic properties of Magneto, MagR and αGFP-TRPV1/GFP-ferritin

Wang, G.·Zhang, P.·Mendu, S. K.·Wang, Y.·Zhang, Y.·Kang, X.·Desai, B. N.·Zhu, J. J.
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Abstract

ABSTRACT AND INTRODUCTIONMagnetic control of neuronal activity offers many obvious advantages over electric, optogenetic and chemogenetic manipulations. A recent series of highly visible papers reported the development of magnetic actuators (i.e., Magneto, MagR and GFP-TRPV1/GFP-ferritin) that appeared to be effective in controlling neuronal firing1-3, yet their action mechanisms seem to conflict with the principles of physics4. We found that neurons expressing Magneto, MagR and GFP-TRPV1/GFP-ferritin did not respond to magnetic stimuli with any membrane depolarization (let alone action potential firing), although these neurons frequently generated spontaneous action potentials. Because the previous study did not establish the precise temporal correlation between magnetic stimuli and action potentials in recorded neurons1-3, the reported magnetically-evoked action potentials are likely to represent mismatched spontaneous firings.

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Wang, G., Zhang, P., Mendu, S. K., Wang, Y., Zhang, Y., Kang, X., Desai, B. N., Zhu, J. J.. 2019-08-27. Revaluation of magnetic properties of Magneto, MagR and αGFP-TRPV1/GFP-ferritin. https://doi.org/10.1101/737254

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