Cytosine but not adenine base editor generates mutations in mice

Lee, H. K.; Smith, H. E.; Liu, C.; Willi, M.; Hennighausen, L.

doi:10.1101/731927

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bioRxiv · 10.1101/731927

Cytosine but not adenine base editor generates mutations in mice

Lee, H. K.·Smith, H. E.·Liu, C.·Willi, M.·Hennighausen, L.

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Abstract

Deaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms and its ultimate success therapeutically depends on its accuracy. Here we have investigated the fidelity of cytosine base editor 4 (BE4) and adenine base editor (ABE) in mouse embryos using unbiased whole genome sequencing of a family-based trio cohort. We demonstrate that BE4-edited mice carry an excess of single-nucleotide variants and deletions compared to ABE-edited mice and controls.

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Lee, H. K., Smith, H. E., Liu, C., Willi, M., Hennighausen, L.. 2019-08-12. Cytosine but not adenine base editor generates mutations in mice. https://doi.org/10.1101/731927

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