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bioRxiv · 10.1101/725465

Spatial and temporal control of CRISPR/Cas9-mediated gene editing delivered via a light-triggered liposome system

Aksoy, Y. A.·Chen, W.·Goldys, E. M.·Deng, W.
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Abstract

The CRISPR-Cas9 and related systems offer a unique genome editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with CRISPR/Cas9 system. We demonstrated its high transfection efficiency, by assessing the targeted knockout of eGFP gene in human HEK293 cells (52.8% knockout). We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). To the best of our knowledge we reported the first proof-of-concept of spatio-temporal control of CRISPR/Cas9 by using light-triggered liposomes in both in vitro and in vivo environment.

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Aksoy, Y. A., Chen, W., Goldys, E. M., Deng, W.. 2019-08-05. Spatial and temporal control of CRISPR/Cas9-mediated gene editing delivered via a light-triggered liposome system. https://doi.org/10.1101/725465

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