Long-term functional culture and in vitro manipulation of adult mouse cardiomyocytes

Callaghan, N. I.; Lee, S.-H.; Hadipour-Lakmehsari, S.; Lee, X. A.; Siraj, M. A.; Driouchi, A.; Yip, C. M.; Husain, M.; Simmons, C. A.; Gramolini, A. O.

doi:10.1101/721514

bioRxiv · 10.1101/721514

Long-term functional culture and in vitro manipulation of adult mouse cardiomyocytes

Callaghan, N. I.·Lee, S.-H.·Hadipour-Lakmehsari, S.·Lee, X. A.·Siraj, M. A.·Driouchi, A.·Yip, C. M.·Husain, M.·Simmons, C. A.·Gramolini, A. O.

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Abstract

Primary adult cardiomyocyte (aCM) culture is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca2+ cycling to enable physiologically-relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations that probe mechanisms underlying myocyte functional performance.

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Callaghan, N. I., Lee, S.-H., Hadipour-Lakmehsari, S., Lee, X. A., Siraj, M. A., Driouchi, A., Yip, C. M., Husain, M., Simmons, C. A., Gramolini, A. O.. 2019-08-01. Long-term functional culture and in vitro manipulation of adult mouse cardiomyocytes. https://doi.org/10.1101/721514

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