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bioRxiv · 10.1101/715482

Template plasmid integration in germline genome-edited cattle.

Norris, A. L.·Lee, S. S.·Greenlees, K. J.·Tadesse, D. A.·Miller, M. F.·Lombardi, H.
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Abstract

We analyzed publicly available whole genome sequencing data from cattle which were germline genome-edited to introduce polledness. Our analysis discovered the unintended heterozygous integration of the plasmid and a second copy of the repair template sequence, at the target site. Our finding underscores the importance of employing screening methods suited to reliably detect the unintended integration of plasmids and multiple template copies.

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Norris, A. L., Lee, S. S., Greenlees, K. J., Tadesse, D. A., Miller, M. F., Lombardi, H.. 2019-07-28. Template plasmid integration in germline genome-edited cattle.. https://doi.org/10.1101/715482

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