Restoring Function After Severe Spinal Cord Injury Through Bioluminescence-Driven Optogenetics

Petersen, E. D.; Sharkey, E. D.; Pal, A.; Shafau, L. O.; Zenchak, J. R.; Pena, A. J.; Aggarwal, A.; Prakash, M.; Hochgeschwender, U.

doi:10.1101/710194

bioRxiv · 10.1101/710194

Restoring Function After Severe Spinal Cord Injury Through Bioluminescence-Driven Optogenetics

Petersen, E. D.·Sharkey, E. D.·Pal, A.·Shafau, L. O.·Zenchak, J. R.·Pena, A. J.·Aggarwal, A.·Prakash, M.·Hochgeschwender, U.

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Abstract

The ability to manipulate specific neuronal populations of the spinal cord following spinal cord injury (SCI) could prove highly beneficial for rehabilitation in patients through maintaining and strengthening still existing neuronal connections and/or facilitating the formation of new connections. A non-invasive and highly specific approach to neuronal stimulation is bioluminescent-optogenetics (BL-OG), where genetically expressed light emitting luciferases are tethered to light sensitive channelrhodopsins (luminopsins, LMO); neurons are activated by the addition of the luciferase substrate coelenterazine (CTZ). This approach utilizes ion channels for current conduction while activating the channels through application of a small chemical compound, thus allowing non-invasive stimulation and recruitment of all targeted neurons. Rats were transduced in the lumbar spinal cord with AAV2/9 to express the excitatory LMO3 under control of a pan-neuronal or motor neuron-specific promoter. A day after contusion injury of the thoracic spine, rats received either CTZ or vehicle every other day for 2 weeks. Activation of either interneuron or motor neuron populations below the level of injury significantly improved locomotor recovery lasting beyond the time of stimulation. Utilizing histological and gene expression methods we identified neuronal plasticity as a likely mechanism underlying the functional recovery. These findings provide a foundation for a rational approach to spinal cord injury rehabilitation, thereby advancing approaches for functional recovery after SCI.

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Petersen, E. D., Sharkey, E. D., Pal, A., Shafau, L. O., Zenchak, J. R., Pena, A. J., Aggarwal, A., Prakash, M., Hochgeschwender, U.. 2019-07-22. Restoring Function After Severe Spinal Cord Injury Through Bioluminescence-Driven Optogenetics. https://doi.org/10.1101/710194

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