Clonal transmission and new mechanism of resistance to trimethoprim-sulfamethoxazole in Stenotrophomonas maltophilia strains isolated in a neonatology unit at Antananarivo, Madagascar, deciphered by whole genome sequence analysis

Rabenandrasana, M. A. N.; Andrianoelina, V.; Bonneault, M.; Herindrainy, P.; Garin, B.; Breurec, S.; Delarocque-Astagneau, E.; Andrianirina, Z. Z.; Enouf, V.; Huynh, B.-T.; Opatowski, L.; Collard, J. M.

doi:10.1101/696765

bioRxiv · 10.1101/696765

Clonal transmission and new mechanism of resistance to trimethoprim-sulfamethoxazole in Stenotrophomonas maltophilia strains isolated in a neonatology unit at Antananarivo, Madagascar, deciphered by whole genome sequence analysis

Rabenandrasana, M. A. N.·Andrianoelina, V.·Bonneault, M.·Herindrainy, P.·Garin, B.·Breurec, S.·Delarocque-Astagneau, E.·Andrianirina, Z. Z.·Enouf, V.·Huynh, B.-T.·Opatowski, L.·Collard, J. M.

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Abstract

Stenotrophomonas maltophilia has been recognized as an emerging multidrug resistant organism in hospital settings due to its resistance to a broad range of antimicrobial agents. These include {beta}-lactams and aminoglycosides, afforded by the existence of intrinsic and acquired resistance mechanisms. Trimethoprim/sulfamethoxazole (SXT) is recommended as one of the best treatment choices against S. maltophilia infections; however increasing resistance to SXT has complicated the treatment. From July 2014 to March 2015, individuals and surfaces from a neonatology ward in Antananarivo, Madagascar, were longitudinally followed to assess the transmission of bacteria resistant to antibiotics between neonates, individuals (parents and nurses) and ward environments. Four S. maltophilia strains were successively isolated from a water-tap (N=1), from feces obtained from a newborn (N=1), and nursing staff (N=2). Antimicrobial susceptibility testing and whole genome sequencing were performed on each isolate. Based on coregenome alignment, all strains were identical and belonged to the new sequence type ST-288. They were resistant to trimethoprim-sulfamethoxazole, carbapenems and intermediate to levofloxacin. Each isolate carried the aadB, strA, strB and sul1 genes located in a class I integron but variants of the dfrA gene were absent. We assessed by PROVEAN analysis the single nucleotide mutations found in folA, folC and folM genes and only the mutation in folA (A114T:GCC[->]ACC) has an effect on the activity of trimethoprim. Our findings demonstrated the prolonged presence of SXT-resistant S. maltophilia in a clinical setting with consecutive transfers from the environment to a newborn and staff based on the isolation dates. We also hypothesized that single nucleotide mutations in folA could be responsible for trimethoprim resistance.

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Rabenandrasana, M. A. N., Andrianoelina, V., Bonneault, M., Herindrainy, P., Garin, B., Breurec, S., Delarocque-Astagneau, E., Andrianirina, Z. Z., Enouf, V., Huynh, B.-T., Opatowski, L., Collard, J. M.. 2019-07-10. Clonal transmission and new mechanism of resistance to trimethoprim-sulfamethoxazole in Stenotrophomonas maltophilia strains isolated in a neonatology unit at Antananarivo, Madagascar, deciphered by whole genome sequence analysis. https://doi.org/10.1101/696765

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