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bioRxiv · 10.1101/695213

Bacterial fucosidase enables the production of Bombay red blood cells

Li, T.·Ye, J.·Wang, L.·Zou, L.·Guo, Y.·Hou, L.·Shen, D.·Cai, X.·Huang, H.·Sun, G.·Chen, L.
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Abstract

We present a method to produce H antigen-deficient red blood cells (RBCs) for transfusion to individuals with anti-H antibodies. A fucosidase from bacteria was heterologously expressed efficiently in E. coli and has been demonstrated to completely remove H antigens on the surface of human RBCs in a facile conversion process. The approach we describe here holds promise for making H-deficient RBCs available for a rare population beyond ABO types.

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Li, T., Ye, J., Wang, L., Zou, L., Guo, Y., Hou, L., Shen, D., Cai, X., Huang, H., Sun, G., Chen, L.. 2019-07-08. Bacterial fucosidase enables the production of Bombay red blood cells. https://doi.org/10.1101/695213

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