Neoteny in visual system development of the spotted unicornfish, Naso brevirostris (Acanthuridae)

Abstract

Ontogenetic changes of the visual system are often correlated to shifts in habitat and feeding behaviour of animals. Coral reef fishes begin their lives in the pelagic zone and then migrate to the reef. This transition of habitat frequently involves a change in diet and light environment as well as major morphological modifications. The spotted unicornfish, Naso brevirostris, is known to shift diet from zooplankton to algae and back to zooplankton when transitioning from larval to juvenile and then to adult stages. Concurrently, N. brevirostris also moves from an open pelagic to a coral-associated habitat before migrating up in the water column when reaching adulthood. Using retinal mapping techniques, we discovered that the distribution and density of ganglion and photoreceptor cells in N. brevirostris do not change with the habitat or the feeding habits of each developmental stage. Instead, fishes showed a neotenic development with a slight change from larval to juvenile stages and not many modifications thereafter. Visual gene expression based on RNA sequencing mirrored this pattern; independent of stage, fishes mainly expressed three cone opsin genes (SWS2B, RH2B, RH2A), with a quantitative difference in the expression of the green opsin genes (RH2A and RH2B) when transitioning from larvae to juveniles. Hence, contrary to the ontogenetic changes found in many animals, the visual system is fixed early on in N. brevirostris development calling for a thorough analysis of visual system development of the reef fish community.