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bioRxiv · 10.1101/681270

The genome design suite: enabling massive in-silico experiments to design genomes.

Chalkley, O.·Purcell, O.·Grierson, C.·Marucci, L.
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Abstract

MotivationComputational biology is a rapidly developing field, and in-silico methods are being developed to aid the design of genomes to create cells with optimised phenotypes. Two barriers to progress are that in-silico methods are often only developed on a particular implementation of a specific model (e.g. COBRA metabolic models) and models with longer simulation time inhibit the large-scale in-silico experiments required to search the vast solution space of genome combinations.\n\nResultsHere we present the genome design suite (PyGDS) which is a suite of Python tools to aid the development of in-silico genome design methods. PyGDS provides a framework with which to implement phenotype optimisation algorithms on computational models across computer clusters. The framework is abstract allowing it to be adapted to utilise different computer clusters, optimisation algorithms, or design goals. It implements an abstract multi-generation algorithm structure allowing algorithms to avoid maximum simulation times on clusters and enabling iterative learning in the algorithm. The initial case study will be genome reduction algorithms on a whole-cell model of Mycoplasma genitalium for a PBS/Torque cluster and a Slurm cluster.\n\nAvailabilityThe genome design suite is written in Python for Linux operating systems and is available from GitHub on a GPL open-source licence.\n\nContacto.chalkley@bristol.ac.uk, lacsg@bristol.ac.uk, and lucia.marucci@bristol.ac.uk.

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Chalkley, O., Purcell, O., Grierson, C., Marucci, L.. 2019-06-24. The genome design suite: enabling massive in-silico experiments to design genomes.. https://doi.org/10.1101/681270

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