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bioRxiv · 10.1101/671503

Diverse noncanonical PAMs recognized by SpCas9 in human cells

Hu, Z.·Wang, D.·Zhang, C.·Wang, S.·Gao, S.·Hou, L.·Wang, H.·Wang, Y.
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Abstract

The CRISPR/Cas9 system derived from Streptococcus pyogenes (SpCas9) provides unprecedented genome editing capabilities, but the potential for off-target mutations limits its application. In addition to NGG protospacer adjacent motif (PAM), off-target mutations are also associated with noncanonical PAMs, which have not yet been systematically evaluated. Here, we developed a highly sensitive approach that allows systematically analyzing PAM sequences in human cells, and identified multiple alternative PAMs recognized by SpCas9.

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Hu, Z., Wang, D., Zhang, C., Wang, S., Gao, S., Hou, L., Wang, H., Wang, Y.. 2019-06-14. Diverse noncanonical PAMs recognized by SpCas9 in human cells. https://doi.org/10.1101/671503

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