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bioRxiv · 10.1101/670927

Olsenella lakotia SW165 sp. nov., an acetate producing obligate anaerobe with a GC rich genome

Wongkuna, S.·Ghimire, S.·Kumar, R.·Antony, L.·Chankhamhaengdecha, S.·Janvilisri, T.·Scaria, J.
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Abstract

A Gram-positive and obligately anaerobic bacterium was isolated from cecal content of feral chickens in Brookings, South Dakota, USA. The microorganism grew at 37-45{degrees} C and pH 6-7.5. This strain produced acetic acid as the primary metabolic end product. Major fatty acids were C12:0, C14:0, C14:0 DMA and summed feature 1 (C13:1 at 12-13 and C14:0 aldehyde). Phylogenetic analyses based on 16S rRNA gene sequence suggested that strain SW165 belongs to the family Atopobiaceae with the closest relatives being Olsenella profusa DSM 13989T (96.33% similarity), Olsenella umbonate DSM 26220T (96.18%) and Olsenella uli DSM 7084T (96.03%). Genome sequencing revealed a genome size of 2.43 Mbp with a G+C content of 67.59 mol%, which is the highest G+C content among members of the genus Olsenella. Phylogenetic and phenotypic comparison indicated that strain SW165 represents a novel species of the genus Olsenella, for which the name Olsenella lakotia sp. nov. is proposed. The type strain is SW165 (=DSM 107283T).

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Wongkuna, S., Ghimire, S., Kumar, R., Antony, L., Chankhamhaengdecha, S., Janvilisri, T., Scaria, J.. 2019-06-13. Olsenella lakotia SW165 sp. nov., an acetate producing obligate anaerobe with a GC rich genome. https://doi.org/10.1101/670927

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