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bioRxiv · 10.1101/667790

Protein structure from experimental evolution

Stiffler, M. A.·Poelwijk, F. J.·Brock, K. P.·Stein, R. R.·Teyra, J.·Sidhu, S. S.·Marks, D. S.·Gauthier, N. P.·Sander, C.
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Abstract

Natural evolution encodes rich information about the structure and function of biomolecules in the genetic record. Previously, statistical analysis of co-variation patterns in natural protein families has enabled the accurate computation of 3D structures. Here, we explored whether similar information can be generated by laboratory evolution, starting from a single gene and performing multiple cycles of mutagenesis and functional selection. We evolved two bacterial antibiotic resistance proteins, {beta}-lactamase PSE1 and acetyltransferase AAC6, and obtained hundreds of thousands of diverse functional sequences. Using evolutionary coupling analysis, we inferred residue interactions in good agreement with contacts in the crystal structures, confirming genetic encoding of structural constraints in the selected sequences. Computational protein folding with contact constraints yielded 3D structures with the same fold as that of natural relatives. Evolution experiments combined with inference of residue interactions from sequence information opens the door to a new experimental method for the determination of protein structures.

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Stiffler, M. A., Poelwijk, F. J., Brock, K. P., Stein, R. R., Teyra, J., Sidhu, S. S., Marks, D. S., Gauthier, N. P., Sander, C.. 2019-06-13. Protein structure from experimental evolution. https://doi.org/10.1101/667790

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