Time machine: Can a dye from 1928 be re-purposed for modern, fluorescence-based detection of amyloid-like fibrils?

Antimonova, O. I.; Grudinina, N. A.; Egorov, V. V.; Ilyin, V. V.; Zabrodskaya, Y. A.; Ramsay, E. S.; Polukeev, V. A.; Shavlovsky, M. M.

doi:10.1101/665364

bioRxiv · 10.1101/665364

Time machine: Can a dye from 1928 be re-purposed for modern, fluorescence-based detection of amyloid-like fibrils?

Antimonova, O. I.·Grudinina, N. A.·Egorov, V. V.·Ilyin, V. V.·Zabrodskaya, Y. A.·Ramsay, E. S.·Polukeev, V. A.·Shavlovsky, M. M.

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Abstract

This manuscript describes the chemical synthesis of a compound similar to fluorene and Congo red, including characterization of its spectral properties. It was shown that the dye, during interaction with amyloid-like fibrils of several proteins (lysozyme, insulin, and beta-2-microglobulin), has the ability to change fluorescent spectrum. In contrast, monomeric forms of these proteins did not induce significant spectral changes.

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Antimonova, O. I., Grudinina, N. A., Egorov, V. V., Ilyin, V. V., Zabrodskaya, Y. A., Ramsay, E. S., Polukeev, V. A., Shavlovsky, M. M.. 2019-06-10. Time machine: Can a dye from 1928 be re-purposed for modern, fluorescence-based detection of amyloid-like fibrils?. https://doi.org/10.1101/665364

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