A rule-based data-informed cellular consensus map of the human mononuclear phagocyte cell space

Guenther, P.; Cirovic, B.; Bassler, K.; Haendler, K.; Becker, M.; Dutertre, C. A.; Bigley, V.; Newell, E. W.; Collin, M.; Ginhoux, F.; Schlitzer, A.; Schultze, J. L.

doi:10.1101/658179

bioRxiv · 10.1101/658179

A rule-based data-informed cellular consensus map of the human mononuclear phagocyte cell space

Guenther, P.·Cirovic, B.·Bassler, K.·Haendler, K.·Becker, M.·Dutertre, C. A.·Bigley, V.·Newell, E. W.·Collin, M.·Ginhoux, F.·Schlitzer, A.·Schultze, J. L.

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Abstract

Single-cell genomic techniques are opening new avenues to understand the basic units of life. Large international efforts, such as those to derive a Human Cell Atlas, are driving progress in this area; here, cellular map generation is key. To expedite the inevitable iterations of these underlying maps, we have developed a rule-based data-informed approach to build next generation cellular consensus maps. Using the human dendritic-cell and monocyte compartment in peripheral blood as an example, we performed computational integration of previous, partially overlapping maps using an approach we termed backmapping, combined with multi-color flow-cytometry and index sorting-based single-cell RNA-sequencing. Our general strategy can be applied to any atlas generation for humans and other species.\n\nGraphical Abstract\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=110 SRC=\"FIGDIR/small/658179v1_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (18K):\norg.highwire.dtl.DTLVardef@c1b7b2org.highwire.dtl.DTLVardef@32b68org.highwire.dtl.DTLVardef@16e5fcorg.highwire.dtl.DTLVardef@1552028_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LIDefining a consensus of the human myeloid cell compartment in peripheral blood\nC_LIO_LI3 monocytes subsets, pDC, cDC1, DC2, DC3 and precursor DC make up the compartment\nC_LIO_LIDistinguish myeloid cell compartment from other cell spaces, e.g. the NK cell space\nC_LIO_LIProviding a generalizable method for building consensus maps for the life sciences\nC_LI

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Guenther, P., Cirovic, B., Bassler, K., Haendler, K., Becker, M., Dutertre, C. A., Bigley, V., Newell, E. W., Collin, M., Ginhoux, F., Schlitzer, A., Schultze, J. L.. 2019-06-03. A rule-based data-informed cellular consensus map of the human mononuclear phagocyte cell space. https://doi.org/10.1101/658179

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