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bioRxiv · 10.1101/655407

Multiplexable fluorescence lifetime imaging (FLIM) probes for Syk and Src-family kinases

Damayanti, N. P.·Jena, S.·Irudayaraj, J. M. K.·Parker, L. L.
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Abstract

Most commonly employed strategies to map kinase activities in live cells require expression of genetically-encoded proteins (e.g. FRET sensors). In this work, we describe development and preliminary application of a set of cell-penetrating, fluorophore labelled peptide substrates for fluorescence lifetime imaging (FLIM) of Abl and Src-family kinase activities. These probes do not rely on FRET pairs or genetically-encoded protein expression. We also demonstrate image-by-image and pixel-by-pixel quantification of probe phosphorylation ratio, suggesting that this strategy will be useful for detailed mapping of single cell and subcellular kinase activity in live cells.

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Damayanti, N. P., Jena, S., Irudayaraj, J. M. K., Parker, L. L.. 2019-05-30. Multiplexable fluorescence lifetime imaging (FLIM) probes for Syk and Src-family kinases. https://doi.org/10.1101/655407

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