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bioRxiv · 10.1101/648501

TGFβ drives mitochondrial dysfunction in peripheral blood NK cells during metastatic breast cancer

Abstract

Natural Killer (NK) cells provide important protection from cancer and are a key requirement for particular immunotherapies. In activated NK cells, a metabolic response towards increased glycolysis and oxidative phosphorylation is crucial for NK cell effector functions. However, there is accumulating evidence that NK cells become dysfunctional during chronic inflammatory diseases, such as human breast cancer. This dysfunction is apparent in peripheral blood NK cells and can impact on normal NK cell immune responses and their effective targeting during immunotherapy. Herein, we demonstrate that prolonged cytokine stimulation combined with metabolic restriction, through inhibition of mTORC1, is sufficient to induce persistent dysfunction in human NK cells. TGF{beta}, also restricted NK cell metabolism and promoted persistent NK cell dysfunction. NK cells from patients with metastatic breast cancer had profound metabolic defects in glycolysis and mitochondrial function, and clear structural differences in NK cell mitochondrial morphology. Importantly, blocking elevated TGF{beta} improved readouts of metabolism and restored IFN{gamma} production in patient NK cells.

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Slattery, K., Zaiatz-Bittencourt, V., Woods, E., Brennan, K., Marks, S., Chew, S., Conroy, M., Goggin, C., Kennedy, J., Finlay, D. K., Gardiner, C. M.. 2019-05-27. TGFβ drives mitochondrial dysfunction in peripheral blood NK cells during metastatic breast cancer. https://doi.org/10.1101/648501

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