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bioRxiv · 10.1101/647065

Two-color nanoscopy of organelles for extended times with HIDE probes

Chu, L.·Tyson, J.·Shaw, J. E.·Rivera-Molina, F.·Koleske, A. J.·Schepartz, A.·Toomre, D.
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Abstract

Performing multi-color nanoscopy for extended times is challenging due to the rapid photobleaching rate of most fluorophores. Here we describe a new fluorophore (Yale-595) and a bio-orthogonal labeling strategy that enables both super-resolution (STED) and 3D confocal imaging of two organelles simultaneously for extended times using high-density environmentally sensitive (HIDE) probes. Because HIDE probes are small, cell-permeant molecules, they can visualize organelle pairs (ER + mitochondria, ER + plasma membrane) in hard-to-transfect cell lines at super-resolution for up to 7 minutes. The extended time domain possible using these new tools reveal novel dynamic nanoscale targeting between organelles.

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Chu, L., Tyson, J., Shaw, J. E., Rivera-Molina, F., Koleske, A. J., Schepartz, A., Toomre, D.. 2019-05-22. Two-color nanoscopy of organelles for extended times with HIDE probes. https://doi.org/10.1101/647065

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