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bioRxiv · 10.1101/645903

Enabling high-accuracy long-read amplicon sequences using unique molecular identifiers and Nanopore sequencing

Karst, S. M.·Ziels, R.·Kirkegaard, R. H.·Albertsen, M.
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Abstract

High-throughput amplicon sequencing of large genomic regions remains challenging for short-read technologies. Here, we report a high-throughput amplicon sequencing approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies or Pacific Biosciences CCS sequencing, yielding high accuracy single-molecule consensus sequences of large genomic regions. Our approach generates amplicon and genomic sequences of >10,000 bp in length with a mean error-rate of 0.0049-0.0006% and chimera rate <0.022%.

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Karst, S. M., Ziels, R., Kirkegaard, R. H., Albertsen, M.. 2019-05-24. Enabling high-accuracy long-read amplicon sequences using unique molecular identifiers and Nanopore sequencing. https://doi.org/10.1101/645903

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