A universal method for gene expression engineering

Lale, R.; Tietze, L.; Nesje, J.; Onsager, I.; Engelhardt, K.; Wong, C. F. A.; Akan, M.; Hummel, N.; Kalinowski, J.; Rueckert, C.; Hohmann-Marriott, M. F.

doi:10.1101/644989

bioRxiv · 10.1101/644989

A universal method for gene expression engineering

Lale, R.·Tietze, L.·Nesje, J.·Onsager, I.·Engelhardt, K.·Wong, C. F. A.·Akan, M.·Hummel, N.·Kalinowski, J.·Rueckert, C.·Hohmann-Marriott, M. F.

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Abstract

The precise expression of genes is one of the foundations of biotechnology. Here we present GeneEE, a straightforward method for generating artificial gene expression systems. We demonstrate that GeneEE segments, containing a 200 nucleotide DNA with random nucleotide composition, can facilitate constitutive and inducible gene expression. To highlight the universal character of our method, we demonstrate GeneEE-mediated gene and protein expression in six bacterial species and Bakers yeast.

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Lale, R., Tietze, L., Nesje, J., Onsager, I., Engelhardt, K., Wong, C. F. A., Akan, M., Hummel, N., Kalinowski, J., Rueckert, C., Hohmann-Marriott, M. F.. 2019-05-23. A universal method for gene expression engineering. https://doi.org/10.1101/644989

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