Small molecule inhibitors of G9a reactivate the maternal PWS genes in Prader-Willi-Syndrome patient derived neural stem cells and differentiated neurons

Wu, H.; Ng, C.; Villegas, V.; Chamberlain, S.; Cacace, A.; Wallace, O.

doi:10.1101/640938

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bioRxiv · 10.1101/640938

Small molecule inhibitors of G9a reactivate the maternal PWS genes in Prader-Willi-Syndrome patient derived neural stem cells and differentiated neurons

Wu, H.·Ng, C.·Villegas, V.·Chamberlain, S.·Cacace, A.·Wallace, O.

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Abstract

Abstract/SummaryPatients with Prader-Willi-Syndrome (PWS) display intellectual impairment, hyperphagia, and various behavioral problems during childhood that converge on a neurologic deficit. The majority of PWS patients have genetic deletions of the paternal 15q11-q13 chromosomal region, with their maternal PWS locus intact but epigenetically silenced by hypermethylation and repressive histone modulation of the PWS imprinting center (PWS-IC). Inhibition of the euchromatin histone methyltransferase G9a by small molecules has been recently reported to reactivate PWS genes in patient fibroblasts and a mouse model. However, it is unknown if inhibition of G9a could have similar effect in human PWS neural cells, the cell types that have direct pathophysiological relevance to PWS. Here, we use neural progenitor cells (NPCs) and cortical excitatory neurons derived from a patient iPSC to model PWS, and quantitatively profile the expression of PWS genes using a NanoString panel. We demonstrated that the methylation of the PWS-IC is stable during neuronal lineage conversion, and that the maternal PWS genes remain silenced in PWS NPCs and neurons. Multiple small molecule inhibitors of G9a activate maternal PWS genes in a dose dependent manner in both NPCs and neurons. In addition, G9a inhibitors induce GNRH1 and HTR2C, two neuronal specific genes that contribute to PWS pathology in neurons. Interestingly, distinct from 5-Azacytidine, G9a inhibition does not induce methylation changes of the maternal PWS-IC, indicating that disruption of the histone repressive complex alone is sufficient to drive an open chromatin state at the PWS-IC that leads to partial reactivation of PWS genes. HighlightsO_LIModeling PWS disease in a dish using patient derived NPCs and neurons C_LIO_LIG9a inhibition activates maternal PWS genes in patient-derived neural cells C_LIO_LIG9a inhibition activates maternal SNORD116 and other PWS genes in patient-derived neurons C_LIO_LIInhibition of G9a induces PWS downstream genes GNRH1 and HTR2C in PWS neurons C_LI

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Wu, H., Ng, C., Villegas, V., Chamberlain, S., Cacace, A., Wallace, O.. 2019-05-17. Small molecule inhibitors of G9a reactivate the maternal PWS genes in Prader-Willi-Syndrome patient derived neural stem cells and differentiated neurons. https://doi.org/10.1101/640938

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