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bioRxiv · 10.1101/640920

Live-cell STED microscopy of mitochondrial cristae

Stephan, T.·Roesch, A.·Riedel, D.·Jakobs, S.
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Abstract

Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They exhibit a smooth outer membrane and a highly convoluted inner membrane that forms invaginations called cristae. Imaging cristae in living cells poses a formidable challenge for light microscopy. Relying on a cell line stably expressing the mitochondrial protein Cox8A fused to the SNAP-tag and using STED (stimulated emission depletion) super-resolution microscopy, we demonstrate the visualization of cristae dynamics in cultivated human cells.

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Stephan, T., Roesch, A., Riedel, D., Jakobs, S.. 2019-05-17. Live-cell STED microscopy of mitochondrial cristae. https://doi.org/10.1101/640920

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