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bioRxiv · 10.1101/639278

Actin chromobody imaging reveals sub-organellar actin dynamics

Schiavon, C. R.·Zhang, T.·Zhao, B.·Andrade, L. R.·Wu, M.·Sung, T.-C.·Dayn, Y.·Feng, J. W.·Quintero, O. A.·Grosse, R.·Manor, U.
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Abstract

The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are difficult to detect with fluorescence microscopy. We developed an approach using fluorescent protein-tagged actin nanobodies targeted to organelle membranes to enable live cell imaging of sub-organellar actin dynamics with unprecedented spatiotemporal resolution. These probes reveal that ER-associated actin drives fission of multiple organelles including mitochondria, endosomes, lysosomes, peroxisomes, and the Golgi.\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=100 SRC=\"FIGDIR/small/639278v2_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (46K):\norg.highwire.dtl.DTLVardef@18851e1org.highwire.dtl.DTLVardef@b7c608org.highwire.dtl.DTLVardef@12a7629org.highwire.dtl.DTLVardef@b39a0a_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Schiavon, C. R., Zhang, T., Zhao, B., Andrade, L. R., Wu, M., Sung, T.-C., Dayn, Y., Feng, J. W., Quintero, O. A., Grosse, R., Manor, U.. 2019-05-15. Actin chromobody imaging reveals sub-organellar actin dynamics. https://doi.org/10.1101/639278

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