Inhibition of ADORA1 attenuates hepatic steatosis by gut microbiota-derived acetic acid from Astragalus polysaccharides

Hong, Y.; Zheng, N.; He, X.; Zhong, J.; Ma, J.; Zhao, A.; Zheng, X.; Gu, Y.; Yao, J.; Li, Y.; Yuan, L.; Lin, M.; Lu, X.; Ping, J.; Song, P.; Li, B.; Han, R.; Sheng, L.; Zhang, W.; Ding, K.; Liu, J.; Jia, W.; Li, H.

doi:10.1101/639195

bioRxiv · 10.1101/639195

Inhibition of ADORA1 attenuates hepatic steatosis by gut microbiota-derived acetic acid from Astragalus polysaccharides

Hong, Y.·Zheng, N.·He, X.·Zhong, J.·Ma, J.·Zhao, A.·Zheng, X.·Gu, Y.·Yao, J.·Li, Y.·Yuan, L.·Lin, M.·Lu, X.·Ping, J.·Song, P.·Li, B.·Han, R.·Sheng, L.·Zhang, W.·Ding, K.·Liu, J.·Jia, W.·Li, H.

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Abstract

The authors have withdrawn this manuscript, because some data could not be replicated. During the process of subsequent revision of the manuscript, we separated the data into two parts. The majority data of the preprint draft, the anti-NAFLD effect of Astragalus polysaccharides (APS) in mice and the contribution of gut microbiota, were independently published in 2021 after substantial revision (doi: https://doi.org/10.1080/19490976.2021.1930874). However, the data on figure 6 (about ARs inhibitors DPCPX orally ameliorating NAFLD in mice, and Adora1 si-RNA data in vitro) could not be fully replicated in subsequent experiments. The most probable reason might be due to the batch differences of mice / cells, or other undetermined factors during experiment. Moreover, we have carried out in-depth studies on the exact role of hepatic A1R on NAFLD formation, which is opposite to our prior assumption. Therefore, we do not wish this work to be cited as reference for the project. We apologize for any inconvenience caused to our readers. Any questions can be addressed to first/corresponding author.

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Hong, Y., Zheng, N., He, X., Zhong, J., Ma, J., Zhao, A., Zheng, X., Gu, Y., Yao, J., Li, Y., Yuan, L., Lin, M., Lu, X., Ping, J., Song, P., Li, B., Han, R., Sheng, L., Zhang, W., Ding, K., Liu, J., Jia, W., Li, H.. 2019-05-17. Inhibition of ADORA1 attenuates hepatic steatosis by gut microbiota-derived acetic acid from Astragalus polysaccharides. https://doi.org/10.1101/639195

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