An Open-Hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses

Russell, C.; Rees, E. J.

doi:10.1101/636977

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bioRxiv · 10.1101/636977

An Open-Hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses

Russell, C.·Rees, E. J.

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Abstract

Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sample outside of the depth of field of the detection axis. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning of a length of coverslips or microscopes in range of the detection objective. We present an open-hardware (1, 2) sample mounting system for light-sheet microscopes using large detection objectives.

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Russell, C., Rees, E. J.. 2019-05-13. An Open-Hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses. https://doi.org/10.1101/636977

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