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bioRxiv · 10.1101/620823

Immunological efficacy of self-assembled nanoparticle anti-mite vaccine

Smith, J.·Kowalski, S.
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Abstract

This report demonstrates the effects of self-assembled nanoparticle anti-caries vaccine Glu-FTH and Glu+ Poly(I:C) (in combination with adjuvant Poly(I:C) and antigen Glu) on specific humoral and mucosal immunity in mice. Mice were randomly divided into 6 groups, and Glu-FTH, Glu, Glu-FTH + Poly (I: C), Glu+Poly (I: C), FTH, and PBS were injected into mice via nasal mucosa. Enzyme-linked immunosorbent assay (ELISA) to detect specific antibody levels in serum and saliva. Results indicate that Glu-FTH, Glu, Glu-FTH+Poly(I:C), Glu+Poly(I:C) can effectively increase anti-Glu IgG levels in mouse serum; Glu+Poly(I:C) and Glu It can effectively increase the level of anti-GlusIgA in mouse saliva. Therefore, we demonstrate that Glu-FTH has a certain immune effect. The combination of adjuvant Poly(I:C) and antigen Glu can induce strong immune response.

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Smith, J., Kowalski, S.. 2019-04-28. Immunological efficacy of self-assembled nanoparticle anti-mite vaccine. https://doi.org/10.1101/620823

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