Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli

Abstract

When nutrients run out, bacteria enter a dormant metabolic state. This low or undetectable metabolic activity helps bacteria to preserve their scant reserves for future, but also diminishes their ability to trace the environment for new growth-promoting substrates. However, neighboring microbial growth is a sure indicator of favorable environment and thus, can serve as a cue for exiting the dormancy. Here we report that for Escherichia coli this cue is the basic peptidoglycan unit (i.e. muropeptide). We show that several forms of muropeptides can stimulate growth resumption of dormant E. coli cells, but the sugar - peptide bond is crucial for activity. We also demonstrate that muropeptides from several different species can induce growth resumption of E. coli and also Pseudomonas aeruginosa. These results, together with the previous identification of muropeptides as germination signal for bacterial spores, makes muropeptides rather universal cue for bacterial growth.