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bioRxiv · 10.1101/602433

Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast

Ünal, E.·King, G. A.·Goodman, J. S.·Chetlapalli, K.·Schick, J. G.·Jorgens, D. M.·McDonald, K. L.
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Abstract

Production of healthy gametes in meiosis relies on the quality control and proper distribution of both nuclear and cytoplasmic contents. Meiotic differentiation naturally eliminates age-induced cellular damage by an unknown mechanism. Using time-lapse fluorescence microscopy in budding yeast, we found that nuclear senescence factors - including protein aggregates, extrachromosomal ribosomal DNA circles, and abnormal nucleolar material - are sequestered away from chromosomes during meiosis II and subsequently eliminated. A similar sequestration and elimination process occurs for the core subunits of the nuclear pore complex in both young and old cells. Nuclear envelope remodeling drives the formation of a membranous compartment containing the sequestered material. Importantly, de novo generation of plasma membrane is required for the sequestration event, preventing the inheritance of long-lived nucleoporins and senescence factors into the newly formed gametes. Our study uncovers a new mechanism of nuclear quality control and provides insight into its function in meiotic cellular rejuvenation.

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Ünal, E., King, G. A., Goodman, J. S., Chetlapalli, K., Schick, J. G., Jorgens, D. M., McDonald, K. L.. 2019-04-08. Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast. https://doi.org/10.1101/602433

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