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bioRxiv · 10.1101/596916

Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture

Panteleev, D.·Pustogarov, N.·Revishchin, A.·Shamadykova, D.·Drozd, S.·Goryanov, S.·Potapov, A.·Pavlova, G.
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Abstract

Nucleolus-oriented protein nucleolin plays a significant role in the life of a normal mammalian cell. However, nucleolin is also actively expressed in cells of malignant tumors. At the same time, its expression in different types of cancer is significantly increased compared with normal cells. It is interesting that nucleolin localization often varies in tumor cells, namely in the cytoplasm and on the cell membrane. This fact is considered to be a poor prognostic indicator. This work is devoted to the study of the distribution of nucleolin in human glioblastoma cells. Glioblastoma is one of the most aggressive malignant tumors with an absolutely unfavorable prognosis. These tumors have a high proliferative potential, but in addition they are often characterized by invasive properties. Research on lineage cells does not let to fully study these properties of glioblastoma, since lineage cells are very different from the actual tumor. In our study, we used two primary cell cultures of human glioblastoma with varying degrees of invasiveness of the original tumors. The main interest was directed at studying the localization of nucleolin and its correlation with the invariability of the N- and C-termini of the corresponding protein. Particular attention was paid to the significance of the unaltered C-terminus of nucleolin for its distribution in the cells of transplanted human glioblastoma cultures derived from patient tissues.\n\nThe aim of this work is to find the relationship between the deformation of the N- or C-terminal sequences of nucleolin and its localization.We showed that in glioblastoma cells, with a high degree of invasion, nucleolin is found in the cytoplasm and close to the cell membrane, and the distribution of nucleolin with undeformed C and N-terminal does not match.

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Panteleev, D., Pustogarov, N., Revishchin, A., Shamadykova, D., Drozd, S., Goryanov, S., Potapov, A., Pavlova, G.. 2019-04-02. Different localization of fluorescently labeled N- and C-termini of nucleolin variants in human glioblastoma cell culture. https://doi.org/10.1101/596916

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