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bioRxiv · 10.1101/588434

AluMine: alignment-free method for the discovery of polymorphic Alu element insertions

Abstract

BackgroundRecently, alignment-free sequence analysis methods have gained popularity in the field of personal genomics. These methods are based on counting frequencies of short k-mer sequences, thus allowing faster and more robust analysis compared to traditional alignment-based methods.\n\nResultsWe have created a fast alignment-free method, AluMine, to analyze polymorphic insertions of Alu elements in the human genome. We tested the method on 2,241 individuals from the Estonian Genome Project and identified 28,962 potential polymorphic Alu element insertions. Each tested individual had on average 1,574 Alu element insertions that were different from those in the reference genome. In addition, we propose an alignment-free genotyping method that uses the frequency of insertion/deletion-specific 32-mer pairs to call the genotype directly from raw sequencing reads. Using this method, the concordance between the predicted and experimentally observed genotypes was 98.7%. The running time of the discovery pipeline is approximately 2 hours per individual. The genotyping of potential polymorphic insertions takes between 0.4 and 4 hours per individual, depending on the hardware configuration.\n\nConclusionsAluMine provides tools that allow discovery of novel Alu element insertions and/or genotyping of known Alu element insertions from personal genomes within few hours.

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BibTeXRIS

Puurand, T., Kukushkina, V., Pajuste, F.-D., Remm, M.. 2019-03-25. AluMine: alignment-free method for the discovery of polymorphic Alu element insertions. https://doi.org/10.1101/588434

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