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bioRxiv · 10.1101/584870

Adaptive optics allows 3D STED-FCS measurements in the cytoplasm of living cells

Barbotin, A.·Galiani, S.·Urbancic, I.·Eggeling, C.·Booth, M.
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Abstract

Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular use for investigations of two dimensional systems like cell membranes, but has so far seen very limited applications to studies of three-dimensional diffusion. One reason for this is the extreme sensitivity of the axial (3D) STED depletion pattern to optical aberrations. We present here an adaptive optics-based correction method that compensates for these aberrations and allows STED-FCS measurements in the cytoplasm of living cells.

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Barbotin, A., Galiani, S., Urbancic, I., Eggeling, C., Booth, M.. 2019-03-21. Adaptive optics allows 3D STED-FCS measurements in the cytoplasm of living cells. https://doi.org/10.1101/584870

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