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bioRxiv · 10.1101/578377

Constitutive Siglec-1 expression by human dendritic cell precursors enables HIV-1 replication and transmission

RUFFIN, N.·Gea-Mallorqui, E.·Brouiller, F.·Jouve, M.·Silvin, A.·See, P.·Dutertre, C.-A.·Ginhoux, F.·Benaroch, P.
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Abstract

Human dendritic cell (DC) lineage has been recently unraveled by high dimensional mapping revealing the existence of a discrete new population of blood circulating DC precursor (pre-DC also referred to as AS DC). Among all blood DC subsets, only pre-DC highly express Siglec-1, a lectin-like receptor able to bind HIV-1. We show that pre-DC are uniquely equipped among blood DC populations to promote HIV-1 replication and dissemination. Pre-DC stands out as the most susceptible DC population to infection by both HIV-1 CXCR4- and CCR5-tropic viral particles in a Siglec-1-dependent manner. HIV-1-infected pre-DC produce new viral progeny and transmit the virus to CD4+ T cells. Upon TLR activation, pre-DC become resistant to HIV-1 fusion and thus to infection and switch to a replication-independent mechanism of virus transfer to activated primary T lymphocytes mediated by Siglec-1. Thus, beside their role in DC ontogeny, blood pre-DC possess stage-specific properties that HIV-1 may exploit for viral spreading and modulation of the immune response.

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RUFFIN, N., Gea-Mallorqui, E., Brouiller, F., Jouve, M., Silvin, A., See, P., Dutertre, C.-A., Ginhoux, F., Benaroch, P.. 2019-03-14. Constitutive Siglec-1 expression by human dendritic cell precursors enables HIV-1 replication and transmission. https://doi.org/10.1101/578377

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