Reduced proteasome activity in the aging brain results in ribosome stoichiometry loss and aggregation

Kelmer Sacramento, E.; Kirkpatrick, J. M.; Mazzetto, M.; Di Sanzo, S.; Caterino, C.; Sanguanini, M.; Papaevgeniou, N.; Lefaki, M.; Childs, D.; Bagnoli, S.; Terzibasi Tozzini, E.; Bartolome, A.; Romanov, N.; Baumgart, M.; Huber, W.; Chondrogianni, N.; Vendruscolo, M.; Cellerino, A.; Ori, A.

doi:10.1101/577478

bioRxiv · 10.1101/577478

Reduced proteasome activity in the aging brain results in ribosome stoichiometry loss and aggregation

Kelmer Sacramento, E.·Kirkpatrick, J. M.·Mazzetto, M.·Di Sanzo, S.·Caterino, C.·Sanguanini, M.·Papaevgeniou, N.·Lefaki, M.·Childs, D.·Bagnoli, S.·Terzibasi Tozzini, E.·Bartolome, A.·Romanov, N.·Baumgart, M.·Huber, W.·Chondrogianni, N.·Vendruscolo, M.·Cellerino, A.·Ori, A.

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Abstract

A progressive loss of protein homeostasis is characteristic of aging and a driver of neurodegeneration. To investigate this process quantitatively, we characterized proteome dynamics during brain aging in the short-lived vertebrate Nothobranchius furzeri combining transcriptomics and proteomics. We detected a progressive reduction in the correlation between protein and mRNA, mainly due to post-transcriptional mechanisms that account for over 40% of the age-regulated proteins. These changes cause a progressive loss of stoichiometry in several protein complexes, including ribosomes, which show impaired assembly / dis-assembly and are enriched in protein aggregates in old brains. Mechanistically, we show that reduction of proteasome activity is an early event during brain aging and is sufficient to induce proteomic signatures of aging and loss of stoichiometry in vivo. Using longitudinal transcriptomic data, we show that the magnitude of early life decline in proteasome levels is the major risk factor for mortality. Our work defines causative events in the aging process that can be targeted to prevent loss of protein homeostasis and delay the onset of age-related neurodegeneration. HighlightsO_LIProgressive loss of stoichiometry affects multiple protein complexes C_LIO_LIRibosomes aggregate in old brains C_LIO_LIPartial reduction of proteasome activity is sufficient to induce loss of stoichiometry C_LIO_LIReduced proteasome levels are a major risk factor for early death in killifish C_LI

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Kelmer Sacramento, E., Kirkpatrick, J. M., Mazzetto, M., Di Sanzo, S., Caterino, C., Sanguanini, M., Papaevgeniou, N., Lefaki, M., Childs, D., Bagnoli, S., Terzibasi Tozzini, E., Bartolome, A., Romanov, N., Baumgart, M., Huber, W., Chondrogianni, N., Vendruscolo, M., Cellerino, A., Ori, A.. 2019-03-16. Reduced proteasome activity in the aging brain results in ribosome stoichiometry loss and aggregation. https://doi.org/10.1101/577478

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